While Sanger sequencing was highly accurate and less labor-intensive than its predecessors, it still required the use of radioactive reagents and manual sequence recording. In 1986, Leroy Hood’s lab at Caltech replaced the radiolabeled ddNTPs with fluorescently labeled nucleotides, using fluorophores that emitted different colors of light for each base. They were now able to run the products of all four reactions on the same gel and have a computer read the sequence by detecting the color of each fluorescent signal as fragments passed through a laser beam.
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В Финляндии предупредили об опасном шаге ЕС против России09:28。下载安装 谷歌浏览器 开启极速安全的 上网之旅。是该领域的重要参考
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This method is cancel safe.